Two different ways ...
. . . are used to ensure the existence of a consistent number of bacteria inside the bowel. The first one consists in administrating first a great quantity of bacteria which will weaken its activity and reduce its quantity during the conservation, but also during the cold storage. During this period, the high mortality rate of probiotics can be possibly caused by their passage into the gastrointestinal tract and therefore we do not know what the real number of intact bacteria that reaches the bowel is. The second method is to ensure that during the product shelf life, the number of bacteria does not change and that at any time this latter can survive to the intestinal tract and ensure the right implantation in the intestinal mucosa carrying out a lacto-fermentative activity. On this point, a study on real-time stability of different Megaflora 9 samples has been carried out, such samples have been stored at ambient temperature (25º C) in its original package. This study has been carried out between 2002 and 2006 using samples to count the number of viable bacteria existing in 3, 6, 12, 24 and 36 months. The results demonstrated that after storing Megaflora 9 at ambient temperature (25ºC) during 36 months, the total number of Megaflora 9 bacteria was still the same as the one at the beginning of its production 1x109 cfu/g (a thousand millions of bacteria per gram). For such reason, Megaflora 9 ensures the existence of 1x109 viable bacteria per gram, during 36 months at ambient temperature, able to survive in the intestinal tract. Megaflora 9 does not need to be preserved at cold temperatures and it combines different Nutritional components: vitamins, minerals, plant extracts, nutraceuticals… etc.
Real time stability
The aim of the activity test is to determine the metabolic activity of the lactic acid bacteria in the product.
General Information
Product(s) : Megaflora 9
Date experiment : 2002-2006
WBI ref number : RTSWIN500
Executed by : Mr. R. Blankenstein
Reviewed by : Mrs. Ir. L. Mulder
Date : 23rd May 2005
Aim of the experiment
The aim of the activity test is to determine the metabolic activity of the lactic acid bacteria in the product.
Background information
In the real time stability test; products are stored up to 3 years and on regular times the total cell count of the product is measured. The product is stored in sealed aluminium sachets or in the original packaging at a temperature of 25°C. Cell count analyses are performed at times 0, 3, 6, 9, 12, 24 and 36 months.
Method
Several batches of Megaflora 9, were stored at 25°C for 2 or 3 years. Analysis of the total cell count was performed at T= 0, 3, 6, 9, 12 and 24 (and 36) months. Batch 02E02.4 and 02J31.1 have been stored for 3 years and batch 03C12.1 for 2 years. For batch 04L07.1 there are stability data available for 12 moths (1 year) now.
Results
The results are shown in the following graph:
